Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

HiScreen^™ columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resins) and designed for method optimization and parameter screening. HiScreen^™ columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The media used in HiScreen^™ columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.Capto^™ Butyl is intended for initial and intermediate step purifications requiring a chromatography medium with Low to medium hydrophobicity. Capto^™ Butyl often works efficiently with rather Low salt concentrations. The mechanism of binding and elution onto the butyl ligand is different from that onto the phenyl ligand, giving a different selectivity. Performance during sample run and selectivity are similar to that of Butyl Sepharose^® 4 Fast Flow. Higher flow rates can be used with Capto^™ Butyl during equilibration, wash and regeneration.

Features and Benefits

Prepacked with Capto^™ Butyl Excellent for method optimization and parameter screening due to the 10 cm bed height Easily connected in series to achieve 20 cm bed height Small bed volume for fast results and minimal sample/buffer consumption Reproducible results, scalable to BioProcess columns packed with the same media using the same flow velocity

General description

Capto^™ Phenyl (High sub) and Capto^™ Butyl are hydrophobic interaction chromatography (HIC) media used in the capture and intermediate stages of protein purification.

Legal Information

Capto is a trademark of Cytiva

Sepharose is a registered trademark of Cytiva

HiScreen is a trademark of Cytiva

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)